美國SKC采樣器Biosampler樣品采集與分析可適用于以下幾種分析法:
生產(chǎn)培養(yǎng)法菌落分析
用生長培養(yǎng)法定量分析和表征空氣培養(yǎng)細(xì)菌和真菌。
液體樣品的一部分被轉(zhuǎn)移到這些微生物是長成菌落計(jì)數(shù)培養(yǎng)基。
微觀分析
顯微鏡分析用于計(jì)數(shù)和有限的鑒定空氣中的細(xì)菌和真菌總數(shù),生物顆粒是區(qū)別于非生物染色技術(shù)。
生化測定
生化法是用來測量如內(nèi)毒素生物化合物。一種化學(xué)物質(zhì)或生物物質(zhì)被加到樣品的一部分。隨后的反應(yīng)形成定量的基礎(chǔ)。
免疫分析法
免疫分析是用于空氣中的過敏原定量(如塵螨、動物皮屑)。它依賴于抗體與特定靶抗原的結(jié)合。其他方法包括熒光、酶和放射免疫分析。
聚合酶鏈反應(yīng)
聚合酶鏈反應(yīng)(PCR)用于篩選特定屬或種的樣本。PCR過程是基于對選定核酸序列的體外復(fù)制。
SKC BioSampler是一個具有(Patented 5,902,385)的率玻璃沖擊瓶捕集裝置的特殊設(shè)計(jì),它需要一臺高流量音速的空氣采樣泵, 用來捕集空氣中微生物(如細(xì)菌、真菌、花粉、病毒)與其副產(chǎn)物如內(nèi)毒素、霉菌毒素及其它碎片,以供進(jìn)一步分析。
analysis
samples collected with the biosampler can be analyzed using:
Growth Culture Analysis
Growth culture analysis is used to quantify and characterize airborne cultural bacteria and fungi.
A portion of the liquid sample is transferred to a culture medium on whic the microorganisms are grown into colonies for counting.
A broad-spectrum of media is gennerally used.
Microscopic Analysis
Microscopic analysis is used for used for the enumeration and limited identification of total airborne bacteria and fungi,Biological particles are differentiated from nonbiological ones by staining techniques.
Biochemical Assay
Biochemical assay is used to measure bilogical compounds such as endotoxins. A chemical or biological substance is added to a portion of the sample
the ensuing reaction forms the basis for quantification
immunoassay
immunoassay is used for the quantification of airborne allergens(e.g.,dust mites and animal dander).
It relies on the binding of antibodies to a specific target antigen. Other methods include fluorescence,enzyme,and radioimmunoassay.
Polymerase Chain Reaction
Polymerase Chain Reaction(pcr) is used to screen samples for a specific genus or species.
the PCR process is based on in-vitro replication of selected nucleic acid sequences.